The study on the interaction between phytosterols and phospholipids in model membranes. Sterols are one of the major components of cellular membranes. Although in mammalian membranes cholesterol is a predominant sterol, in the human organism plant sterols (phytosterols) can also be found. Phytosterols, especially if present in concentrations higher than normal (phytosterolemia), may strongly affect membrane properties. In this work, we studied phytosterol–phospholipid interactions in mixed Langmuir monolayers serving as model membranes.
Investigated were two phytosterols, . The phospholipids had different polar heads, different length and saturation of their hydrocarbon chains. The interactions between molecules in mixed sterol/phospholipid films were characterized with the mean area per molecule (A1.
The effect of the sterols on the molecular organization of the phospholipid monolayers was analyzed based on the compression modulus values. It was found that the incorporation of the phytosterols into the phospholipid monolayers increased their condensation. The plant sterols revealed higher affinity towards phosphatidylcholines as compared to phosphatidylethanolamines. The phytosterols interacted more strongly with phospholipids possessing longer and saturated chains. Moreover, both the length and the saturation of the phosphatidylcholines influenced the stoichiometry of the most stable complexes. Our results, compared with those presented previously for cholesterol/phospholipid monolayers, allowed us to draw a conclusion that the structure of sterol (cholesterol, .
IAPP fiber-formation kinetics and disruption of DOPC/DPPC LUVs. Via the diet are dissolved in the lipid portion of. DPPC) and cholesterol. Sigma-Aldrich offers Sigma-C8667, Cholesterol for your research needs. Find product specific information including CAS, MSDS, protocols and references. The Role of Cholesterol in Driving IAPP-Membrane.
Namely, the strongest interactions were found for cholesterol/phospholipids mixtures, while the weakest for mixed systems containing stigmasterol.
- Solubility Limits of Cholesterol, Lanosterol, Ergosterol, Stigmasterol, and
- The structures of DMPC and DPPC bilayers in unilamellar liposomes, in the presence of 33.3 mol% cholesterol or the plant sterol
Diet Doctor is the world. Our goal is empowering people everywhere to revolutionize their health. DOPC, 1,2-dioleoyl phosphatidylcholine; DPPC.